| 质粒名称: | pLOVE empty vector |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 15948 |
| 存储实验室: | Miguel Ramalho-Santos |
|
载体骨架基本信息 |
|
| 载体名称: | pLOVE (for Lentiviral Over-Expression) |
| 载体出品公司: | -- |
| 载体抗性: | 氯霉素和氨苄青霉素 |
| 空载体大小: | 8263 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 30 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gateway克隆 |
| 5' 克隆位点: | attR1(未破坏) |
| 3' 克隆位点: | attR2 (未破坏) |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pLOVE empty vector was a gift from Miguel Ramalho-Santos (Addgene plasmid # 15948 ; http://n2t.net/addgene:15948 ; RRID:Addgene_15948) |
| 参考文献部分: | Generation of Induced Pluripotent Stem Cells in the Absence of Drug Selection. Blelloch R, Venere M, Yen J, Ramalho-Santos M. Cell Stem Cell. 2007 Sep 13. ():. 10.1016/j.stem.2007.08.008 PubMed 18371358 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLOVE empty vector 8263 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pLOVE empty vector
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8263)
AUTHORS Blelloch R, Venere M, Yen J, Ramalho-Santos M
TITLE Generation of Induced Pluripotent Stem Cells in the Absence of Drug
Selection.
JOURNAL Cell Stem Cell. 2007 Sep 13. ():.
PUBMED 18371358
REFERENCE 2 (bases 1 to 8263)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8263
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(56..75)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 247..626
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 628..826
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
LTR 844..1024
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1071..1196
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1695..1928
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2455..2572
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 2638..2941
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2942..3145
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
protein_bind 3147..3165
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 3168..3186
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 3227..3351
/gene="mutant version of attR"
/label=attR1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter 3376..3406
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
CDS 3460..4119
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind complement(3526..3545)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
CDS 4461..4766
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 4645..4664
/label=ccdB-fwd
/note="ccdB gene, forward primer"
protein_bind complement(4807..4931)
/gene="mutant version of attR"
/label=attR2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
CDS 4984..5025
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
protein_bind 5063..5096
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
misc_feature 5152..5740
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5205..5225)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5623..5634)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(5743..5759)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5744..5760)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 6269..6449
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
rep_origin complement(6511..7099)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6591..6610)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7270..8130)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7893..7912
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8131..8235)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gcttaagcgg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
61 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
241 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
301 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
1021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
1081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
1141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
1201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
1261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
1321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
1381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
1441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
1501 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct
1561 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
1621 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
1681 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
1741 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
1801 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
1861 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
1921 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
1981 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
2041 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
2101 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
2161 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
2221 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
2281 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
2341 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
2401 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa
2461 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
2521 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
2581 ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctagc agttccgcgt
2641 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
2701 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
2761 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
2821 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
2881 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
2941 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
3001 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
3061 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
3121 gtgggaggtc tatataagca gagctctccc tatcagtgat agagatctcc ctatcagtga
3181 tagagatcgt cgactagtcc agtgtggtgg aattctgcag atatcaacaa gtttgtacaa
3241 aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca
3301 taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat ggcggccgca
3361 ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg gattttgagt
3421 taggatccgt cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga
3481 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca
3541 gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc
3601 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg
3661 aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt
3721 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt
3781 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac
3841 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc
3901 aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc
3961 gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg
4021 gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg tcggcagaat gcttaatgaa
4081 ttacaacagt actgcgatga gtggcagggc ggggcgtaaa gatctggatc cggcttacta
4141 aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact
4201 gatatgtata cccgaagtat gtcaaaaaga ggtgtgctat gaagcagcgt attacagtga
4261 cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct
4321 ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga
4381 aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga
4441 cgagaacagg gactggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt
4501 atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga
4561 tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg
4621 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct
4681 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca
4741 ttaacctgat gttctgggga atataaatgt caggctccct tatacacagc cagtctgcag
4801 gtcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc
4861 aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc agctttcttg
4921 tacaaagtgg ttgatatcca gcacagtggc ggccgctcga gtctagaggg cccgcggttc
4981 gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac cggttagtaa
5041 tgacaattcg tcgagggacc taataacttc gtatagcata cattatacga agttatacat
5101 gtttaagggt tccggttcca ctaggtacaa ttcgatatca agcttatcga taatcaacct
5161 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
5221 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
5281 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
5341 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
5401 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
5461 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
5521 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
5581 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
5641 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
5701 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
5761 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
5821 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt
5881 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
5941 ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat
6001 ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc cagggatcag
6061 atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga
6121 agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga
6181 tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat
6241 ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg
6301 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
6361 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
6421 cttttagtca gtgtggaaaa tctctagcag catgtgagca aaaggccagc aaaaggccag
6481 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
6541 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
6601 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
6661 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
6721 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
6781 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
6841 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
6901 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
6961 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
7021 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
7081 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
7141 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
7201 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
7261 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
7321 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
7381 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
7441 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
7501 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
7561 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
7621 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
7681 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
7741 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
7801 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
7861 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
7921 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
7981 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
8041 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
8101 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
8161 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
8221 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gac
//
